Journal: Molecules

Article Title: Synthesis and Pharmacological Effects of Diosgenin–Betulinic Acid Conjugates

doi: 10.3390/molecules25153546

Figure Lengend Snippet: Cytotoxicity screening tests (IC 50 values (μM), 72 h).

Article Snippet: The compounds 1 – 8 were subjected to the cytotoxicity screening tests on cells of human T-lymphoblastic leukemia (CEM), cells of human breast adenocarcinoma (MCF7), cells of human cervical cancer (HeLa), and human colon carcinoma (HCT 116 cancer cell lines), using normal human fibroblasts (BJ) as reference cell lines.

Techniques:

Journal: ACS Omega

Article Title: Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates

doi: 10.1021/acsomega.3c03387

Figure Lengend Snippet: Plots of expected enhancement rates in DNA dsb in HeLa cells (A) × (C) shown in Table vs enhancement rates in cytotoxicity against HeLa cells (B) for 1 P1–3 (red line) and 1M 1–3 (purple line). The slopes of the red and blue lines are 0.44 and 0.053, respectively.

Article Snippet: Prof. Kitagishi provided HeLa (human cervical cancer) cells used in this study (Doshisha University, Kyoto, Japan).

Techniques:

Journal: ACS Omega

Article Title: Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates

doi: 10.1021/acsomega.3c03387

Figure Lengend Snippet: Confocal microscopic images of 1 P1–3 (200 μM) in HeLa cells in the dark on 1 h incubation. Bright-field images (A, E, I). Blue fluorescence indicates the fluorescence of 1 P1 (B), 1 P2 (F), and 1 P3 (J) (λ ex = 405 nm). Red fluorescence is mitochondrial staining by Mito Tracker Deep Red FM (50 nM) (Thermo Fisher) (C, G, K) (λ ex = 640 nm). (D, H, L) Overlay images of panels (A)–(C), (E)–(G), and (I)–(K), respectively. The scale bar is 20 μm.

Article Snippet: Prof. Kitagishi provided HeLa (human cervical cancer) cells used in this study (Doshisha University, Kyoto, Japan).

Techniques: Incubation, Fluorescence, Staining

Journal: ACS Omega

Article Title: Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates

doi: 10.1021/acsomega.3c03387

Figure Lengend Snippet: In Vitro Cytotoxicity of 1 , 1 X , and Cisplatin against Various Cells in MTT Assay (48 h) (Mean ± SD)

Article Snippet: Prof. Kitagishi provided HeLa (human cervical cancer) cells used in this study (Doshisha University, Kyoto, Japan).

Techniques: In Vitro, MTT Assay

Journal: ACS Omega

Article Title: Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates

doi: 10.1021/acsomega.3c03387

Figure Lengend Snippet: Data for the Plot of Expected Enhancement Rates ( 1 X / 1 ) in DNA dsb in HeLa Cells (A) × (C) vs Enhancement Rates ( 1 X / 1 ) in Cytotoxicity against HeLa Cells (B) a

Article Snippet: Prof. Kitagishi provided HeLa (human cervical cancer) cells used in this study (Doshisha University, Kyoto, Japan).

Techniques:

Journal: PLoS Pathogens

Article Title: HIV-1 Vpr-Induced Apoptosis Is Cell Cycle Dependent and Requires Bax but Not ANT

doi: 10.1371/journal.ppat.0020127

Figure Lengend Snippet: (A) HeLa cells were transfected with nonspecific (NS) siRNA or siRNA targeted to ATR, or ATM as indicated. At 48 h post-transfection, cells were either treated with 25 μM etoposide, treated with 25 μM MNNG, mock-transduced, or transduced with pHR-VPR-R. Additionally, cells transfected with siRNAs targeted to Bax or ANT were transduced with pHR-VPR-R or mock-transduced (lower left dot plots). Cells from each treatment were assayed for caspase activity as in B. (B) Cells treated as in (A) were harvested at specified time points post-transduction and assayed for caspase activity. (C) Cells treated as in (A) were stained with DAPI, and the results were quantified by microscopy. (D) Cells treated with the indicated siRNAs were lysed and analyzed by Western blot to verify knockdown efficiency. (E) Primary human CD4 + lymphocytes were infected with DHIV3, infected with DHIV3ΔVPR, or mock-infected. At 48 h postinfection, cells in each treatment were lysed and assayed for protein concentration. Equal amounts of protein from each treatment were incubated with Bax6A7 monoclonal antibody. Antibody–protein complexes were precipitated with agarose beads and boiled and then subjected to Western blot analysis with a polyclonal antibody to Bax. (F) HeLa cells treated with the indicated siRNAs and transduced with pHR-VPR or mock-transduced were lysed; reactivity to Bax6A7 antibody was assayed as described in (E).

Article Snippet: For experiments in which cell synchronization or the use of siRNAs was to be employed, we used the human cervical cancer cell line HeLa, which was maintained in Dulbecco's modified Eagle's medium (Cambrex BioScience (formerly BioWhittaker), http://www.cambrex.com ), supplemented with 10% FCS and 2 mM l -glutamine.

Techniques: Transfection, Transduction, Activity Assay, Staining, Microscopy, Western Blot, Knockdown, Infection, Protein Concentration, Incubation